EnRoute stands for Enhancing Resilience of urban ecosystems through green infrastructure. EnRoute is a project of the European Commission in the framework of the EU Biodiversity Strategy and the Green Infrastructure Strategy. EnRoute provides scientific knowledge of how urban ecosystems can support urban planning at different stages of policy and for various spatial scales and how to help policy-making for sustainable cities. It aims to promote the application of urban green infrastructure at local level and delivers guidance on the creation, management and governance of urban green infrastructure. Importantly, it illustrates how collaboration between and across different policy levels can lead to concrete green infrastructure policy setting.